In an impassioned Instagram post, The Big Bang Star slammed the zoo for killing Harambe, calling it just another “senseless” act.
“…but once again, another senseless horrendous animal being killed over people not using their brains.”
The gorilla was shot but zoo keepers at the weekend after a four year old boy accidentally climbed through fencing and fell into the enclosure.
Footage shows the little boy being dragged through water while onlookers – including his mother- watch on in horror.
Cuoco believes people have completely misrepresented the behaviour of the male gorilla, who she believes did not deserve to die.
Kaley is a fierce animal activist.
"If you watch the footage, you see this gorgeous animal holding that child's hand. Do with that what you will," she wrote.
The actress also went as far to say that Harambe was probably better off dead than alive in the zoo.
"As sad as this makes me, a part of me is happy for that amazing creature doesn't have to live in captivity another day."
Cuoco didn't expect for her post to go unnoticed and and so called on readers to "bring on the hate!!!!"
"I'm sure I will get tons of backlash (per usual) I mean let's be honest, I wear the wrong sweatpants and the entire world has something to say about it."
Despite her declaration to bring on the backlash, Cuoco has been widely supported, with many people opting to either blame the zoo for mishandling the incident or the parents for not watching the child.
"The parents are to be blamed for not watching their child. The child could have been killed in the 15 foot fall before any gorilla was involved. Take responsibility for your children people," one user wrote.
Want to know more about Harmabe and the story? (Post continues after video.)
Another commented, "Totally agree with you! We only have less that 900 gorillas in this world, all due to our egotistical decisions. We humans are destroying this planet."
This isn't the first time Cuoco - who is an animal activist - has spoken out about animal mistreatment.
She previously called "humanity a joke" after two lions were reportedly shot dead by zookeepers after a man jumped into the enclosure and he was subsequently mauled.
"I know the may I saw, the more trouble I will get into but seriously what is this world coming to?? Humanity is a joke. If you are that stupid, you deserve everything that happens to you."