Video shows the moment two families are booted from Jetstar plane over a seating mishap.

Two families were kicked off a Jetstar flight to Adelaide for refusing to go back to their assigned seats after moving to be together.

A video posted on Facebook shows the group being removed from the plane at Bali’s Denpasar airport by flight attendants before take-off late Tuesday night.

Jetstar said the families had refused to take their assigned seats, but passengers aboard the plane said the two fathers were merely questioning why they had not been allocated seats with the rest of their family.

Passenger Vincent Bamforth posted a video online showing a couple and their young daughter packing up their hand luggage and being escorted from the plane.

Mr Bamforth wrote that a young boy “was bawling his eyes out” as his family exited the aircraft, although this moment was not captured on film.

“Jetstar you performed poorly, over exercised your power and should be shamed internationally,” he wrote.

“Your captain is irresponsible and lacked due care and showed complete ignorance.”

Fellow passengers also claimed the cabin crew had overreacted.

“Two families were kicked off the plane last night at 10.30pm for simply asking why the dads were not placed near the families,” a passenger wrote.

“The hostess complained to the manager hostess who then came and spoke to them about their behaviour and how a hostess shouldn’t be spoken to like that.

“Neither families showed any disrespect and the hostess and they still kicked them off the plane with young children over nothing. There are plenty of witnesses to say they were in fact not being rude and simply were asking a question.”

The couple packed up their things before being escorted from the plane. (Image via Facebook/Vincent Bamforth)

A Jetstar spokeswoman told MailOnline the captain decided to remove the passengers from the flight because they refused to move from other people's seats.

"We had customers board a flight from Bali to Adelaide overnight who had taken other passengers seats and refused to move to their allocated seats."

"The passengers refused to follow numerous requests from our crew so the captain decided that they would not travel on that flight.

"Like all airlines, our customers are allocated specific seats and we can't have a situation where passengers sit wherever they like. This causes disruptions to other customers and delays the flight departing."

The spokeswoman said the two families were booked onto another flight on Wednesday morning, free of charge.

Jetstar did not cover their accommodation for the night, according to the Daily Mail.